Children's Healthcare of Atlanta

3 articles published in JoVE

• Neuroscience

  
  Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury Rowan O. Brothers*¹, Sara Bitarafan*^2,3, Alyssa F. Pybus^1,3, Levi B. Wood*^1,2,3, Erin M. Buckley*^{1,4,5 1}Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, ³Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, ⁴Department of Pediatrics, Emory University School of Medicine, ⁵ This protocol presents methods to characterize the neuroinflammatory and hemodynamic response to mild traumatic brain injury and to integrate these data as part of a multivariate systems analysis using partial least squares regression.

• Biology

  
  A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry M. Edward Quach^1,2, Anum K. Syed^1,2, Renhao Li^{1,2 1} We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor pair.

• Bioengineering

  
  Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases David R. Myers*^1,2,3,4, Yumiko Sakurai*^1,2,3,4, Reginald Tran^1,2,3,4, Byungwook Ahn^1,2,3,4, Elaissa Trybus Hardy^1,2,3,4, Robert Mannino^1,2,3,4, Ashley Kita^1,2,3,4, Michelle Tsai^1,2,3,4, Wilbur A. Lam^{1,2,3,4 1}Department of Pediatrics, Emory University School of Medicine, ²Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ³Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta, ⁴Winship Cancer Institute of Emory University A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.