Egg Safety and Quality Research 1 article published in JoVE Environment Experimental Column Setup for Studying Anaerobic Biogeochemical Interactions Between Iron (Oxy)Hydroxides, Trace Elements, and Bacteria Jennifer Hellal1, André Burnol2, Aude Locatelli1,3, Fabienne Battaglia-Brunet1 1Environmental Biogeochemistry and Water Quality Unit, BRGM, 2Risks and Risk Prevention Division, BRGM, 3Egg Safety & Quality Research Fate and speciation of arsenic and mercury in aquifers are closely related to physio-chemical conditions and microbial activity. Here, we present an original experimental column setup that mimics an aquifer and enables a better understanding of trace element biogeochemistry in anoxic conditions. Two examples are presented, combining geochemical and microbiological approaches.