GA-PCOM 1 article published in JoVE Biology Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology Christopher Thompson1, Katherine Keck2, Abigail Hielscher2 1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 2Department of Biomedical Sciences, GA-PCOM Here, we present a protocol for the isolation of whole, intact mouse mammary glands to investigate extracellular matrix (ECM) expression and ductal morphology. Mouse #4 abdominal glands were extracted from 8-10 week old female nulliparous mice, fixed in neutral buffered formalin, sectioned and stained using immunohistochemistry for ECM proteins.
