Mercy College of Health Sciences 1 article published in JoVE Biology Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters Victor Babich1,2, Matthew K. Henry1, Francesca Di Sole1 1Physiology and Pharmacology Department, Des Moines University, 2School of Liberal Arts and Sciences, Mercy College of Health Sciences This manuscript describes the applications of proton-selective electrodes and patch clamping methods to measure the activity of proton transport systems. These methods overcome some limitations of techniques commonly used to study proton transport activity, such as moderate sensitivity, time resolution and insufficient intracellular milieu control.
