Feinberg School of Medicine, Northwestern University 1 article published in JoVE Biology Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons. Wen Lu*1, Urko del Castillo*1,2, Vladimir I. Gelfand1 1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 2IKERBASQUE, Basque Foundation for Science Drosophila S2 cells and cultured neurons are great systems for imaging of motor-driven organelle transport in vivo. Here we describe detailed protocols for culturing both cell types, their imaging and analysis of transport.
