Illinois State University 2 articles published in JoVE Biochemistry Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor Sadia Sultana1, Greg M. Anderson1, Kevin Pierre Hoffmann1, Jan-Ulrik Dahl1 1School of Biological Sciences, Illinois State University This protocol describes the extraction and visualization of aggregated and soluble proteins from Escherichia coli after treatment with a proteotoxic antimicrobial. Following this procedure allows a qualitative comparison of protein aggregate formation in vivo in different bacterial strains and/or between treatments. Behavior Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature Yun Chang1, Alan Ewert2, Lisa M. Kamendulis3, Barbara A. Hocevar3 1School of Kinesiology and Recreation, Illinois State University, 2Department of Recreation, Park, and Tourism Studies, Indiana University, 3Department pf Environmental and Occupational Health, School of Public Health-Bloomington, Indiana University The purpose of this paper is to identify changes in stress levels after visitation to three different settings and to describe the methods used in identifying stress levels based on measures of salivary cortisol, α-amylase, and a psychological self-report instrument.