Scientific Designs 1 article published in JoVE Bioengineering Spatial Temporal Analysis of Fieldwise Flow in Microvasculature Sherry G. Clendenon1,2, Xiao Fu1,3, Robert A. Von Hoene1, Jeffrey L. Clendenon4, James P. Sluka1,2, Seth Winfree5, Henry Mang5, Michelle Martinez5, Adele Filson5, James E. Klaunig6, James A. Glazier1,2, Kenneth W. Dunn5 1Biocomplexity Institute, Indiana University, 2Department of Intelligent Systems Engineering, Indiana University, 3Department of Physics, Indiana University, 4Scientific Designs, 5Department of Medicine, Indiana University, 6School of Public Health, Indiana University To quantify microvascular flow from high speed capillary flow image sequences, we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow) software. Across the full image field and over time, STAFF evaluates flow velocities and generates a sequence of color-coded spatial maps for visualization and tabular output for quantitative analyses.
