USDA-ARS, Hard Winter Wheat Genetics Research Unit 1 article published in JoVE Genetics A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel Ling Huang*1, Xiaohui Deng*1, Ronghua Li1, Yanshi Xia1, Guihua Bai2, Kadambot H.M. Siddique3, Peiguo Guo1 1College of Life Sciences, Guangzhou University, 2Hard Winter Wheat Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, 3The UWA Institute of Agriculture, University of Western Australia Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.
