University of Kansas View Institution's Website 7 articles published in JoVE Cancer Research Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging Siddharth Subham*1,2,3, John D. Jeppson*1, Benjamin K. Gibbs4, Jacqueline Babai2, Riza Alker1, Andrew K. Godwin4,5,6, David Akhavan1,2,3 1Department of Radiation Oncology, University of Kansas Cancer Center, 2Department of Cancer Biology, University of Kansas Cancer Center, 3BioEngineering Program, University of Kansas, 4Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine, University of Kansas Medical Center A protocol to evaluate quantitative tumor cell killing by Jurkat cells expressing chimeric antigen receptor (CAR) targeting single tumor antigen. This protocol can be used as a screening platform for rapid optimization of CAR hinge constructs prior to confirmation in peripheral blood-derived T cells. Engineering Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs Hooman Hosseini1, Feng Guo2, Reza Barati Ghahfarokhi1, Saman A. Aryana2 1Department of Chemical and Petroleum Engineering, University of Kansas, 2Department of Chemical Engineering, University of Wyoming This paper describes a protocol along with a comparative study of two microfluidic fabrication techniques, namely photolithography/wet-etching/thermal-bonding and Selective Laser-induced Etching (SLE), that are suitable for high-pressure conditions. These techniques constitute enabling platforms for direct observation of fluid flow in surrogate permeable media and fractured systems under reservoir conditions. Behavior Central and Divided Visual Field Presentation of Emotional Images to Measure Hemispheric Differences in Motivated Attention Aminda J. O'Hare1, Ruth Ann Atchley2, Keith M. Young3 1Department of Psychology, University of Massachusetts Dartmouth, 2Department of Psychology, University of Kansas, 3Department of Psychology, University of Minnesota Duluth This study compared central versus divided visual field presentations of emotional images to assess differences in motivated attention between the two hemispheres. The late positive potential (LPP) was recorded using electroencephalography (EEG) and event-related potentials (ERPs) methodologies to assess motivated attention. Environment Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions Brian A. Pace1, Helen M. Alexander2, D. Jason Emry3, Kristin L. Mercer1 1Department of Horticulture and Crop Science, Ohio State University, 2Department of Ecology and Evolutionary Biology, University of Kansas, 3Department of Biology, Washburn University Here we present a protocol for assessing seed survivorship, germination and dormancy under field conditions using buried, labeled seed strips and tetrazolium chloride (TZ) viability testing. Neuroscience Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor Limei Ma1, Sachiko Haga-Yamanaka1, Qingfeng Elden Yu1, Qiang Qiu1, SangSeong Kim1, C. Ron Yu1,2 1Stowers Institute for Medical Research, 2Department of Anatomy and Cell Biology, The University of Kansas School of Medicine The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli. Biology A Protocol for Computer-Based Protein Structure and Function Prediction Ambrish Roy1,2, Dong Xu1, Jonathan Poisson1, Yang Zhang1,2 1Center for Computational Medicine and Bioinformatics, University of Michigan, 2Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and functions. Medicine Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice Nikki Cheng1, Diana L. Lambert1 1Department of Pathology, University of Kansas Medical Center In this report, we demonstrate a system to isolate and culture donor cells from the mouse mammary gland, and orthotopically transplant these cells in recipient mice to analyze stromal: epithelial interactions during mammary tumor development.