River Hill Ranch 1 article published in JoVE Biochemistry Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies K. Martin Chow1, Sidney W. Whiteheart1, Jeffrey R. Smiley2, Savita Sharma1, Kathy Boaz2, Meggie J. Coleman2, Alvina Maynard3, Louis B. Hersh1, Craig W. Vander Kooi1 1Department of Molecular and Cellular Biochemistry, University of Kentucky, 2Division of Laboratory Animal Resources, University of Kentucky, 3River Hill Ranch A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.