Murray State University 2 articles published in JoVE Developmental Biology Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae Dena R. Hammond-Weinberger1, Gary T. ZeRuth1 1Department of Biological Sciences, Murray State University Here, we present a protocol for fluorescent antibody-mediated detection of proteins in whole preparations of zebrafish embryos and larvae. Biology An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos Ming Gao1, Patrick McCluskey1, Sudan N. Loganathan1, Alexey L. Arkov1 1Department of Biological Sciences, Murray State University Multi-component protein complexes play crucial roles during cellular function and development. Here we describe a method used to isolate native protein complexes from Drosophila embryos after in vivo crosslinking followed by purification of the crosslinked complexes for subsequent structure-function analysis.