USDA-ARS Food and Feed Safety Research Unit 1 article published in JoVE Environment Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L. Kanniah Rajasekaran1, Ronald J. Sayler2, Rajtilak Majumdar1, Christine M. Sickler1, Jeffrey W. Cary1 1Southern Regional Research Center, USDA-ARS, Food and Feed Safety Research Unit, 2Department of Plant Pathology, University of Arkansas Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein. Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.
