University of Massachusetts Boston 5 articles published in JoVE Medicine Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing Teresa T. Liu*1,2, Allison C. Rodgers*3, Tristan M. Nicholson4, Jill A. Macoska5,6, Paul C. Marker6,7, Chad M. Vezina6,8, Dale E. Bjorling6,8, Alejandro Roldan-Alzate2,9,10, Diego Hernando10,11, Granville L. Lloyd12, Timothy A. Hacker3, William A. Ricke1,6 1Department of Urology, University of Wisconsin-Madison, 2K12 Kure, University of Wisconsin-Madison, 3Cardiovascular Research Center, Department of Medicine, University of Wisconsin-Madison, 4Department of Urology, University of Washington, 5University of Massachusetts Boston, 6U54 George M. O'Brien Center, University of Wisconsin-Madison, 7College of Pharmacy, University of Wisconsin-Madison, 8School of Veterinary Medicine, University of Wisconsin-Madison, 9Department of Mechanical Engineering, University of Wisconsin-Madison, 10Department of Radiology, University of Wisconsin-Madison, 11Department of Medical Physics, University of Wisconsin-Madison, 12Rocky Mountain Regional VA Medical Center, University of Colorado We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD). Developmental Biology Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments Liu Yang1, Sayantanee Paul1, Sarah DuBois-Coyne1, Phillip Kyriakakis2, Alexey Veraksa1 1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS). Biology Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format Scott M. Paulissen1, Linda S. Huang1 1Department of Biology, University of Massachusetts Boston Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format. Behavior Assessment and Evaluation of the High Risk Neonate: The NICU Network Neurobehavioral Scale Barry M. Lester1,2, Lynne Andreozzi-Fontaine1,2, Edward Tronick3, Rosemarie Bigsby1,2 1Center for the Study of Children at Risk, Alpert Medical School, Brown University, 2Women & Infants Hospital of Rhode Island, 3University of Massachusetts, Boston The NICU Network Neurobehavioral Scale (NNNS) was developed as an assessment for the at-risk infant. The purpose of this article is to describe the NNNS, provide video examples of the NNNS procedures and discuss the ways in which the exam has been used. Bioengineering Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology Dustin P. Jones*1, William Hanna*1, Hamid El-Hamidi1, Jonathan P. Celli1 1Department of Physics, University of Massachusetts Boston Particle-tracking microrheology can be used to non-destructively quantify and spatially map changes in extracellular matrix mechanical properties in 3D tumor models.
