US Food and Drug Administration 2 articles published in JoVE Developmental Biology Live Cell Imaging of Chromosome Segregation During Mitosis Prajakta Varadkar1, Kazuyo Takeda2, Brenton McCright1 1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system. Immunology and Infection High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes Lynnsey A. Renn1, Terence C. Theisen1, Maria B. Navarro1, Viraj P. Mane1, Lynnsie M. Schramm1, Kevin D. Kirschman1, Giulia Fabozzi1, Philippa Hillyer1, Montserrat Puig2, Daniela Verthelyi2, Ronald L. Rabin1 1Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Center for Drug Evaluation and Research, US Food and Drug Administration This protocol describes a high-throughput qRT-PCR assay for the analysis of type I and III IFN expression signatures. The assay discriminates single base pair differences between the highly similar transcripts of these genes. Through batch assembly and robotic pipetting, the assays are consistent and reproducible.
