Institute for Bioscience and Biotechnology Research 2 articles published in JoVE Genetics Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis Isabelle Louradour1, Kashinath Ghosh1, Ehud Inbar1, David L. Sacks1, Channa Aluvihare2, Robert A. Harrell II2 1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research This protocol details the steps of CRISPR/Cas9 targeted mutagenesis in sand flies: embryo collection, injection, insect rearing, and identification as well as selection of mutations of interest. Immunology and Infection Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay Ashley E. Nazario-Toole1,2, Louisa P. Wu2,3 1Department of Biology, University of Maryland, 2Department of Cell Biology and Molecular Genetics, University of Maryland, 3Institute for Bioscience and Biotechnology Research, University of Maryland This protocol describes an in vivo phagocytosis assay in adult Drosophila melanogaster to quantify phagocyte recognition and clearance of microbial infections.
