Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute 1 article published in JoVE Genetics Reusable Single Cell for Iterative Epigenomic Analyses Hidetaka Ohnuki1, David J. Venzon2, Alexei Lobanov3,4, Giovanna Tosato1 1Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 3CCR Collaborative Bioinformatics Resource (CCBR), Center for Cancer Research, National Cancer Institute, National Institutes of Health, 4Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute The present protocol describes a single-cell method for iterative epigenomic analyses using a reusable single cell. The reusable single cell allows analyses of multiple epigenetic marks in the same single cell and statistical validation of the results.
