University of Maryland Institute for Bioscience and Biotechnology Research 1 article published in JoVE Biology Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis Arvind Sharma1, Michael Pham1, Robert A. Harrell II2, Andrew B. Nuss3, Monika Gulia-Nuss1 1Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 2Insect Transformation Facility, University of Maryland Institute for Bioscience and Biotechnology Research, 3Department of Agriculture, Veterinary, and Rangeland Sciences, University of Nevada, Reno The present protocol describes a method for injecting tick embryos. Embryo injection is the preferred technique for genetic manipulation to generate transgenic lines.
