St John Providence-Providence Park Hospital 1 article published in JoVE Developmental Biology Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta Naimisha Beeravolu1,2, Christina McKee1,2, Ali Alamri1,2, Sasha Mikhael3, Christina Brown1,2, Mick Perez-Cruet2,4, G. Rasul Chaudhry1,2 1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
