Children's Hospitals and Clinics of Minnesota 1 article published in JoVE Medicine Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine Robert A. Kaiser1,2, Shennen A. Mao1, Jaime Glorioso3, Bruce Amiot1, Clara T. Nicolas1, Kari L. Allen1, Zeji Du1, Caitlin J VanLith1, Raymond D. Hickey1, Scott L. Nyberg1, Joseph B. Lillegard1,2,4 1Department of Surgery, Mayo Clinic, 2 This protocol is intended to describe porcine hepatocyte isolation and ex vivo gene delivery to cure models of metabolic diseases via autologous cell transplantation. Although this particular model enjoys unique advantages that favor successful therapy, the application is a relevant foundation to address additional diseases and indications.