North Carolina State University and The University of North Carolina - Chapel Hill 5 articles published in JoVE Bioengineering Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells Madelyn VanBlunk1, Pritha Agarwalla1,2, Sharda Pandit1,2, Yevgeny Brudno1,2,3 1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute, North Carolina State University, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction. Bioengineering Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments Seema Nandi1,2, Ashley C. Brown1,2 1Joint Department of Biomedical Engineering, North Carolina State University and The University of North Carolina - Chapel Hill, 2Comparative Medicine Institute, North Carolina State University The goal of this protocol is to evaluate the effect of pro- and anti-migratory factors on cell migration within a three-dimensional fibrin matrix. Biology Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell Diego Bohórquez1, Fariha Haque2, Satish Medicetty3, Rodger A. Liddle1 1Department of Medicine, Duke University Medical Center, 2Department of Chemistry, University of North Carolina - Chapel Hill, 3Renovo Neural Incorporated Here, we introduce a method, cocem3D, to unveil the ultrastructure of a specific cell in its native tissue by bridging confocal and serial block-face scanning electron microscopy. Bioengineering Engineering Platform and Experimental Protocol for Design and Evaluation of a Neurally-controlled Powered Transfemoral Prosthesis Fan Zhang1, Ming Liu1, Stephen Harper2,3, Michael Lee3, He Huang1 1Joint Department of Biomedical Engineering, North Carolina State University & University of North Carolina at Chapel Hill, 2Department of Physical Medicine and Rehabilitation, University of North Carolina School of Medicine, 3Atlantic Prosthetics & Orthotics, LLC Neural-machine interfaces (NMI) have been developed to identify the user's locomotion mode. These NMIs are potentially useful for neural control of powered artificial legs, but have not been fully demonstrated. This paper presented (1) our designed engineering platform for easy implementation and development of neural control for powered lower limb prostheses and (2) an experimental setup and protocol in a laboratory environment to evaluate neurally-controlled artificial legs on patients with lower limb amputations safely and efficiently. Bioengineering Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape Darryl A. Boyd1, Andre A. Adams1, Michael A. Daniele1, Frances S. Ligler1,2 1Center for Bio/Molecular Science & Engineering, US Naval Research Laboratory, 2Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina at Chapel Hill Two adjacent fluids passing through a grooved microfluidic channel can be directed to form a sheath around a prepolymer core; thereby determining both shape and cross-section. Photoinitiated polymerization, such as thiol click chemistry, is well suited for rapidly solidifying the core fluid into a microfiber with predetermined size and shape.