Wake Forest Univeristy Health Sciences 2 articles published in JoVE Bioengineering Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink Aleksander Skardal1, Mahesh Devarasetty1, Hyun-Wook Kang1, Young-Joon Seol1, Steven D. Forsythe1, Colin Bishop1, Thomas Shupe1, Shay Soker1, Anthony Atala1 1Wake Forest Institute for Regenerative Medicine, Wake Forest Univeristy Health Sciences We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications. Medicine Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications Sean V. Murphy1, Amritha Kidyoor1, Tanya Reid1, Anthony Atala1, Euan M. Wallace2, Rebecca Lim2 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, 2The Ritchie Centre, Monash Institute of Medical Research, Monash University We describe a protocol to isolate and culture human amnion epithelial cells (hAECs) using animal product-free reagents in accordance with current good manufacturing practices (cGMP) guidelines.
