Wake Forest Institute for Regenerative Medicine 2 articles published in JoVE Biology Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography Rebecca Lim1,2, Marcus J. Zavou1, Phillipa-Louise Milton3, Siow Teng Chan1, Jean L. Tan1, Hayley Dickinson1,2, Sean V. Murphy4, Graham Jenkin1,2, Euan M. Wallace1,2 1The Ritchie Centre, Monash Institute of Medical Research, 2Department of Obstetrics and Gynaecology, Monash Medical Centre, 3Animal Resource Centre, Perth, Australia, 4Wake Forest Institute for Regenerative Medicine The assessment of respiratory physiology has traditionally relied upon techniques, which require restraint or sedation of the animal. Unrestrained whole-body plethysmography, however, provides precise, non-invasive, quantitative analysis of respiratory physiology in animal models. In addition, the technique allows repeated respiratory assessment of mice allowing for longitudinal studies. Medicine Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions Erika L. Spaeth1, Christopher M. Booth2, Frank C. Marini1,2 1Department of Leukemia, MD Anderson Cancer Center, 2Wake Forest Baptist Medical Center, Institute for Regenerative Medicine Complex tissue masses, from organs to tumors, are composed of various cellular elements. We elucidated the contribution of cellular phenotypes within a tissue utilizing multi-labeled fluorescent transgenic mice in combination with multiparameter immunofluorescent staining followed by spectral unmixing to decipher cell origin as well as cell characteristics based on protein expression.
