National Institutes of Health 2 articles published in JoVE Developmental Biology Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells Kilsoo Jeon1, Kyeyoon Park2, Anton M. Jetten1 1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health Presented here is a protocol for the generation of a single-cell culture of human embryonic stem cells and their subsequent differentiation into neural progenitor cells. The protocol is simple, robust, scalable, and suitable for drug screening and regenerative medicine applications. Cancer Research Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay Yukitomo Arao1, Kenneth S. Korach1 1Reproductive Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH) We present a method for analyzing the 4-hydroxy-tamoxifen-dependent estrogen receptor alpha ligand-binding domain dimerization activity using the mammalian two-hybrid assay.
