Protochips, Inc. 4 articles published in JoVE Biology A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management Madeline Dressel Dukes1, Nynke Albertine Krans1, Katherine Marusak1, Stamp Walden1, Tim Eldred1, Alan Franks1, Ben Larson1, Yaofeng Guo1, David Nackashi1, John Damiano1 1Protochips, Inc. Here, we present a protocol to utilize machine-vision software to stabilize dynamic processes during TEM imaging, while concurrently indexing multiple streams of metadata to each image into a navigable timeline. We demonstrate how this platform enables automated calibration and mapping of the electron dose over the course of an experiment. Biochemistry Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice Liza-Anastasia DiCecco*1,2, Samantha Berry*1, G. M. Jonaid*1,3, Maria J. Solares1,4, Liam Kaylor1,4, Jennifer L. Gray5, Carol Bator6, William J. Dearnaley1, Michael Spilman7, Madeline J. Dressel-Dukes8, Kathryn Grandfield2, Sarah M. McDonald Esstman9, Deborah F. Kelly1,5,6 1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy. Engineering Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope Kinga A. Unocic1, Dale K. Hensley1, Franklin S. Walden2, Wilbur C. Bigelow3, Michael B. Griffin4, Susan E. Habas4, Raymond R. Unocic1, Lawrence F. Allard5 1Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, 2Protochips Inc., 3Department of Materials Science & Engineering, University of Michigan, 4Catalytic Carbon Transformation & Scale-up, National Renewable Energy Laboratory, 5Materials Science & Technology Division, Oak Ridge National Laboratory Here, we present a protocol for performing in situ TEM closed-cell gas reaction experiments while detailing several commonly used sample preparation methods. Bioengineering In situ TEM of Biological Assemblies in Liquid Madeline J. Dukes*1, Brian L. Gilmore*2, Justin R. Tanner*2, Sarah M. McDonald2, Deborah F. Kelly2 1Applications Science, Protochips, Inc., 2Virginia Tech Carilion Research Institute Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron microscope.
