Rutgers University-Camden 1 article published in JoVE Bioengineering Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles Matthew Ratti1, Joseph J. Naddeo1, Julianne C. Griepenburg1, Sean M. O'Malley1,2, Daniel M. Bubb1,2, Eric A. Klein2,3 1Physics Department, Rutgers University-Camden, 2Center for Computational and Integrative Biology, Rutgers University-Camden, 3Biology Department, Rutgers University-Camden The antimicrobial properties of metals such as copper and silver have been recognized for centuries. This protocol describes pulsed laser-ablation in liquids, a method of synthesizing metal nanoparticles that provides the ability to fine tune the properties of these nanoparticles to optimize their antimicrobial effects.