Rutgers Biomedical and Health Sciences 5 articles published in JoVE Bioengineering Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture Patrick Erickson1, Aneesha Doshi2, Gunjan Jetley2, Param Amin2, Aamena Mejevdiwala2, Ashna Patel2, Raphaela Bento2, Biju Parekkadan2,3 1Department of Chemical and Biochemical Engineering, Rutgers University, 2Department of Biomedical Engineering, Rutgers University, 3Department of Medicine, Rutgers Biomedical Health Sciences This paper presents a method to construct and operate a low-cost, multichannel perfusion cell culture system for measuring the dynamics of secretion and absorption rates of solutes in cellular processes. The system can also expose cells to dynamic stimulus profiles. Medicine Employing the Forced Oscillation Technique for the Assessment of Respiratory Mechanics in Adults Wei Qian1,2, Avani Desai3, Jennifer H. Therkorn1, Jacquelyn C. Klein-Adams1, Anays M. Sotolongo1,2, Michael J. Falvo1,2 1Airborne Hazards and Burn Pits Center of Excellence, War Related Illness and Injury Study Center, VA NJ Health Care System, 2Rutgers New Jersey Medical School, Rutgers Biomedical and Health Sciences, 3Vassar College As the use of forced oscillation technique (FOT) is increasingly utilized to characterize respiratory mechanics, there is a need to standardize methods with respect to nascent technical guidelines and various manufacturer's recommendations. A detailed protocol is provided including FOT assessment and interpretation for two cases to facilitate the standardization of methods. Developmental Biology Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction AnnJosette Ramirez1,2, Sophie Astrof1 1Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School, Rutgers Biomedical and Health Sciences Here, we describe a protocol to visualize and analyze the pharyngeal arch arteries 3, 4, and 6 of mouse embryos using whole-mount immunofluorescence, tissue clearing, confocal microscopy, and 3D reconstruction. Medicine An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells Lauren S. Sherman1,2, Alexandra Condé-Green3, Yahaira Naaldijk1, Edward S. Lee3, Pranela Rameshwar1,2 1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School, Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School, Rutgers Biomedical and Health Sciences This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic. Neuroscience Stochastic Noise Application for the Assessment of Medial Vestibular Nucleus Neuron Sensitivity In Vitro Sebastian P. Stefani1,2, Paul P. Breen3, Jorge M. Serrador4, Aaron J. Camp1,2 1Discipline of Physiology, Sydney Medical School, University of Sydney, 2Bosch Institute, Sydney Medical School, University of Sydney, 3The MARCS Institute, Western Sydney University, 4Department of Pharmacology, Physiology & Neuroscience, Rutgers Biomedical and Health Sciences Galvanic vestibular stimulation in humans exhibits improvements in vestibular function. However, it is unknown how these effects occur. Here, we describe how to apply sinusoidal and stochastic electrical noise and evaluate appropriate stimulus amplitudes in individual medial vestibular nucleus neurons in the C57BL/6 mouse.
