Hackensack Meridian Health Center 2 articles published in JoVE Immunology and Infection A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice Michael T. McGuire*1, Andrea Z. Tuckett*2, Faith Myint2, Johannes L. Zakrzewski2,3,4 1Department of Radiology, Hackensack University Medical Center, 2Center for Discovery and Innovation, Hackensack Meridian Health, 3Department of Pediatrics, Hackensack University Medical Center, 4Department of Oncology, Georgetown University The present protocol describes an interventional radiology procedure established for intrathymic injection in mice to avoid the risk of open surgery and improve the accuracy of blind percutaneous injections. Immunology and Infection Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes Wei Hu1,2, Xiang Song1, Haibo Yu1, Jingyu Sun2, Yong Zhao1 1Center for Discovery and Innovation, Hackensack Meridian Health Center, 2Department of Chemistry and Chemistry Biology, Stevens Institute of Technology Exosome application is an emerging tool for drug development and regenerative medicine. We establish an exosome isolation protocol with high purity to isolate exosomes from novel identified stem cells called CB-SC for mechanistic studies. We also coculture CB-SC-derived exosomes with human monocytes, leading to their differentiation into phenotypically distinct macrophages.
