Sandia National Laboratories View Institution's Website 6 articles published in JoVE Engineering Co-localizing Kelvin Probe Force Microscopy with Other Microscopies and Spectroscopies: Selected Applications in Corrosion Characterization of Alloys Olivia O. Maryon*1, Corey M. Efaw*1, Frank W. DelRio2, Elton Graugnard1,3, Michael F. Hurley1,3, Paul H. Davis1,3 1Micron School of Materials Science & Engineering, Boise State University, 2Material, Physical, and Chemical Sciences Center, Sandia National Laboratories, 3Center for Advanced Energy Studies Kelvin probe force microscopy (KPFM) measures surface topography and differences in surface potential, while scanning electron microscopy (SEM) and associated spectroscopies can elucidate surface morphology, composition, crystallinity, and crystallographic orientation. Accordingly, the co-localization of SEM with KPFM can provide insight into the effects of nanoscale composition and surface structure on corrosion. Bioengineering Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay Zachary I. Imam1, George D. Bachand1 1Center for Integrated Nanotechnologies, Sandia National Laboratories This protocol describes a process for fabricating lipid nanotube networks using gliding kinesin motility in conjunction with giant unilamellar lipid vesicles. Bioengineering Forming Giant-sized Polymersomes Using Gel-assisted Rehydration Adrienne C. Greene1, Darryl Y. Sasaki2, George D. Bachand1 1Center for Integrated Nanotechnologies, Sandia National Laboratories, 2Biological and Engineering Sciences, Sandia National Laboratories We present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies. Engineering Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes Nils Hansen1, Scott A. Skeen1, Hope A. Michelsen1, Kevin R. Wilson2, Katharina Kohse-Höinghaus3 1Combustion Research Facility, Sandia National Laboratories, 2Chemical Sciences Division, Advanced Light Source, Lawrence Berkeley National Laboratory, 3Physikalische Chemie I, Universität Bielefeld Gas sampling from laboratory-scale flames with online analysis of all species by mass spectrometry is a powerful method to investigate the complex mixture of chemical compounds occurring during combustion processes. Coupled with tunable soft ionization via synchrotron-generated vacuum-ultraviolet radiation, this technique provides isomer-resolved information and potentially fragment-free mass spectra. Bioengineering A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments Anupama Sinha1, Mais J. Jebrail2, Hanyoup Kim2,3, Kamlesh D. Patel4, Steven S. Branda2 1Department of Systems Biology, Sandia National Laboratories, 2Department of Biotechnology and Bioengineering, Sandia National Laboratories, 3Canon U.S. Life Sciences, 4Department of Advanced Systems Engineering and Deployment, Sandia National Laboratories We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or reagents. Bioengineering Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing Philip R. Miller1,2, Shelby A. Skoog1, Thayne L. Edwards2, David R. Wheeler2, Xiaoyin Xiao2, Susan M. Brozik2, Ronen Polsky2, Roger J. Narayan1 1Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2Department of Biosensors and Nanomaterials, Sandia National Laboratories This article details the construction of a multiplexed microneedle-based sensor. The device is being developed for in situ sampling and electrochemical analysis of multiple analytes in a rapid and selective manner. We envision clinical medicine and biomedical research uses for these microneedle-based sensors.
