Acoustic Ecology Institute 1 article published in JoVE Environment Technique for Studying Arthropod and Microbial Communities within Tree Tissues Nicholas C Aflitto1, Richard W Hofstetter1, Reagan McGuire1, David D Dunn2, Kristen A Potter1 1School of Forestry, Northern Arizona University, 2Acoustic Ecology Institute We provide a technique to preserve intact tree phloem and prepare it for observation. We create an apparatus called a phloem sandwich that allows for the introduction and observation of arthropods, microbes, and other organisms that inhabit phloem tissues.