University of Nevada-Reno 1 article published in JoVE Biochemistry Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography Alexander J. Bolt*1, Linh D. Do*1, Maryam Raeeszadeh-Sarmazdeh1 1Department of Chemical & Materials Engineering, University of Nevada, Reno His-tag purification, dialysis, and activation are employed to increase yields of soluble, active matrix metalloproteinase-3 catalytic domain protein expression in bacteria. Protein fractions are analyzed via SDS-PAGE gels.