Stony Brook Medicine 4 articles published in JoVE Medicine Bedside Ultrasound for Guiding Fluid Removal in Patients with Pulmonary Edema: The Reverse-FALLS Protocol Danielle Nichole O’Hara1, Vikram Chabra2, Sahar Ahmad2 1Stony Brook University School of Medicine, 2Stony Brook University Medical Center, Stony Brook University School of Medicine We describe an imaging protocol that enables the clinician to visualize, in real-time, the patient's intravascular and extravascular space volumes and set diuresis and fluid removal parameters accordingly for the safe and efficient treatment of pulmonary edema. Neuroscience Remotely Supervised Transcranial Direct Current Stimulation: An Update on Safety and Tolerability Michael T. Shaw1, Margaret Kasschau2, Bryan Dobbs1, Natalie Pawlak1, William Pau1, Kathleen Sherman1, Marom Bikson3, Abhishek Datta4, Leigh E. Charvet1 1New York University, Langone Medical Center, 2Stony Brook Medicine, 3City College of New York, 4Soterix Medical This manuscript provides an updated remote supervision protocol that enables participation in transcranial direct current stimulation (tDCS) clinical trials while receiving treatment sessions from home. The protocol has been successfully piloted in both patients with multiple sclerosis and Parkinson's disease. Biology Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses Agnieszka B. Bialkowska*1, Amr M. Ghaleb*1, Mandayam O. Nandan1, Vincent W. Yang1,2 1Department of Medicine, Stony Brook University School of Medicine, 2Department of Physiology & Biophysics, Stony Brook University School of Medicine Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues. Medicine A Protocol for the Use of Remotely-Supervised Transcranial Direct Current Stimulation (tDCS) in Multiple Sclerosis (MS) Margaret Kasschau1,2, Kathleen Sherman1,2, Lamia Haider2, Ariana Frontario1,2, Michael Shaw1,2, Abhishek Datta3, Marom Bikson4, Leigh Charvet1,2 1Multiple Sclerosis Comprehensive Care Center, Department of Neurology, NYU Langone Medical Center, 2Department of Neurology, Stony Brook Medicine, 3Soterix Medical, Inc, 4Department of Biomedical Engineering, The City College of New York The goal of this pilot study is to describe a protocol for the remotely-supervised delivery of transcranial direct current stimulation (tDCS) so that the procedure maintains standards of in-clinic practice, including safety, reproducibility, and tolerability. The feasibility of this protocol was tested in participants with multiple sclerosis (MS).