New York University Abu Dhabi 2 articles published in JoVE Biology High-Throughput Metabolic Profiling for Model Refinements of Microalgae Amnah Alzahmi1,2, Sarah Daakour1, Diana Charles El Assal3, Bushra S. Dohai3, Amphun Chaiboonchoe3, Weiqi Fu3,4, David R. Nelson1, Alexandra Mystikou1, Kourosh Salehi-Ashtiani1,3 1Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Research Institute, 2Department of Biology, United Arab Emirates University, 3Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math, New York University Abu Dhabi, 4Department of Marine Science, Ocean College, Zhejiang University This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model. Engineering Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles Xi Wei1,2, Abeer Syed1, Pan Mao3, Jongyoon Han4, Yong-Ak Song1,2 1Division of Engineering, New York University Abu Dhabi (NYUAD), 2Department of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, 3Newomics, Inc., 4Department of Electrical Engineering and Computer Science, Department of Biological Engineering, MIT We propose a simple self-assembly technique of silica colloidal nanoparticles to create a nanofluidic junction between two microchannels in polydimethylsiloxane (PDMS). Using this technique, a nanoporous bead membrane with a pore size down to ~45 nm was built inside a microchannel and applied to electrokinetic preconcentration of DNA samples.