The Michael J. Fox Foundation for Parkinson's Research 2 articles published in JoVE Biochemistry Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation Ying Fan*1, Francesca Tonelli*1, Shalini Padmanabhan2, Marco A.S. Baptista2, Lindsey Riley2, Danielle Smith3, Connie Marras4, Andrew Howden5, Dario R. Alessi1, Esther Sammler1,6 1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2 Mutations in the leucine rich repeat kinase 2 gene (LRRK2) cause hereditary Parkinson’s disease. We have developed an easy and robust method for assessing LRRK2-controlled phosphorylation of Rab10 in human peripheral blood neutrophils. This may help identify individuals with increased LRRK2 kinase pathway activity. Neuroscience Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo Joseph R. Patterson1, Nicole K. Polinski2, Megan F. Duffy1,3, Christopher J. Kemp1, Kelvin C. Luk4, Laura A. Volpicelli-Daley5, Nicholas M. Kanaan1, Caryl E. Sortwell1,3,6 1Department of Translational Science and Molecular Medicine, Michigan State University, 2 The goal of this article is to outline the steps required for the generation of fibrils from monomeric alpha-synuclein, subsequent quality control, and use of the preformed fibrils in vivo.
