State University of New York (SUNY) Upstate Medical University 1 article published in JoVE Biochemistry Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy Jessica L. Henty-Ridilla1 1Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology, State University of New York (SUNY) Upstate Medical University This protocol is a guide for visualizing dynamic actin and microtubules using an in vitro total internal fluorescence (TIRF) microscopy assay.