University of Toledo College of Medicine and Life Sciences 2 articles published in JoVE Immunology and Infection Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice Yi Yao1,2, Yun Liu1,3, Akira Takashima1 1Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, 2Department of Internal Medicine, Yale University School of Medicine, 3Department of Pathophysiology, Southern Medical University (China) This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals. Biology Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy Daxiang Li1, Jian Wu1, Yan Bai1, Xiaochen Zhao2, Lijun Liu1 1Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, 2Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.
