Dean McGee Eye Institute 1 article published in JoVE Immunology and Infection Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis Md Huzzatul Mursalin*1,2, Erin Livingston*1, Phillip S. Coburn2,3, Frederick C. Miller4, Roger Astley2,3, Michelle C. Callegan1,2,3 1Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 2Department of Ophthalmology, Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine, University of Oklahoma Health Sciences Center We describe here a method of intravitreal injection and subsequent bacterial quantitation in mouse model of bacterial endophthalmitis. This protocol can be extended for measuring host immune responses and bacterial and host gene expression.
