Lewis & Clark College 2 articles published in JoVE Developmental Biology Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging Zoe T. Cook1, Nicole L. Brockway1, Tamily A. Weissman1 1Biology Department, Lewis & Clark College In vivo imaging is a powerful tool that can be used to investigate the cellular mechanisms underlying nervous system development. Here we describe a technique for using time-lapse confocal microscopy to visualize large numbers of multicolor Brainbow-labeled cells in real time within the developing zebrafish nervous system. Neuroscience Super-resolution Imaging of Neuronal Dense-core Vesicles Bethe A. Scalettar1,2, Daniel Shaver2, Stefanie Kaech3, Janis E. Lochner2,4 1Department of Physics, Lewis & Clark College, 2Program in Biochemistry and Molecular Biology, Lewis & Clark College, 3Jungers Center for Neuroscience Research, Oregon Health & Science University, 4Department of Chemistry, Lewis & Clark College We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.
