Magee Womens Research Institute 1 article published in JoVE Neuroscience Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media Madison T. Jones1, Samantha W. Manioci1, Ashley E. Russell1,2 1Department of Biology, School of Science, Penn State Erie, The Behrend College, 2Magee Womens Research Institute—Allied Member The protocol here demonstrates that extracellular vesicles can be adequately separated from conditioned cell culture media using size exclusion chromatography.