University of Puerto Rico 3 articles published in JoVE Developmental Biology Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution Leighton H. Duncan1,5, Mark W. Moyle1,5, Lin Shao1,5, Titas Sengupta1,5, Richard Ikegami1,5, Abhishek Kumar4,5, Min Guo4,5, Ryan Christensen4,5, Anthony Santella2,5, Zhirong Bao2,5, Hari Shroff4,5, William Mohler3,5, Daniel A. Colón-Ramos1,5,6 1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico Here, we present a combinatorial approach using high-resolution microscopy, computational tools, and single-cell labeling in living C. elegans embryos to understand single cell dynamics during neurodevelopment. Neuroscience Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions Yancy Ferrer-Acosta*1, Maxine N. Gonzalez-Vega*1, David E. Rivera-Aponte2, Solianne M. Martinez-Jimenez1, Antonio H. Martins3 1Department of Neuroscience, School of Medicine, Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine, Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus, University of Puerto Rico Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions. Neuroscience A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila Rebeccah J. Katzenberger1, Carin A. Loewen2, R. Tayler Bockstruck1, Mikal A. Woods3, Barry Ganetzky2, David A. Wassarman1 1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Department of Genetics, University of Wisconsin-Madison, 3Department of Natural Sciences, University of Puerto Rico-Aguadilla Here we describe a method to inflict closed head traumatic brain injury (TBI) in Drosophila. This method provides a gateway to investigate the cellular and molecular mechanisms that underlie TBI pathologies using the vast array of experimental tools and techniques available for flies.
