College of Charleston 2 articles published in JoVE Chemistry Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry Tuguldur T. Odbadrakh1, Ariel G. Gale1, Benjamin T. Ball1, Berhane Temelso2, George C. Shields1 1Department of Chemistry, Furman University, 2College of Charleston The atmospheric concentrations of weakly bound molecular clusters can be computed from the thermochemical properties of low energy structures found through a multi-step configurational sampling methodology utilizing a genetic algorithm and semi-empirical and ab initio quantum chemistry. Genetics Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays Sophia Emetu*1, Morgan Troiano*1, Jacob Goldmintz*1, Jensen Tomberlin1, Simon Grelet2, Philip H. Howe2, Christopher Korey3, Renaud Geslain1 1Laboratory of tRNA Biology, Department of Biology, College of Charleston, 2Department of Biochemistry and Molecular Biology, MUSC, 3Department of Biology, College of Charleston We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.
