Texas Heart Institute 2 articles published in JoVE Bioengineering Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation Doris A. Taylor1, Luiz C. Sampaio1, Rafael Cabello1, Abdelmotagaly Elgalad1, Rohan Parikh1, R Patrick Wood2, Kevin A. Myer2, Alvin T. Yeh3, Po-Feng Lee1 1Regenerative Medicine Research, Texas Heart Institute, 2Lifegift Organ Donation Center, 3Biomedical Engineering, Texas A&M University This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow. Medicine Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging Xiaobing Liu*1,2, Toya Terry*1, Su Pan1, Zhongwei Yang1, James T. Willerson1, Richard A. F. Dixon1, Qi Liu1 1The Texas Heart Institute at St. Luke's Episcopal Hospital, 2Department of Vascular Surgery, The Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University We show here the in vivo insertion of an osmotic pump for constant local drug delivery and the creation of hindlimb ischemia in a mouse model. Moreover, the hindlimb vasculature is perfused with Microfil, a silicone radiopaque agent, to prepare for micro-computed tomography (micro-CT) imaging.