Baylor Scott and White Health 1 article published in JoVE Medicine Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction Himakarnika Alluri1,2, Chinchusha Anasooya Shaji2, Matthew L. Davis1,2, Binu Tharakan1,2 1Department of Surgery, Texas A&M University Health Science Center College of Medicine, 2Department of Surgery, Baylor Scott & White Health Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.
