Texas A&M College of Dentistry 2 articles published in JoVE Developmental Biology Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing Changchun Dong*1, Bikash Lamichhane*1, Yongxu Zhang1, Xiaofang Wang1 1Department of Biomedical Sciences, Texas A&M School of Dentistry The mouse incisor contains valuable label-retaining cells in its stem cell niche. We have a novel way to unbiasedly detect and quantify the label-retaining cells; our study used EdU labeling and a 3D reconstruction approach after PEGASOS tissue clearing of the mandible. Bioengineering Propagation of Dental and Respiratory Cells and Organs in Microgravity Mirali Pandya1, Wei Ma1,2, Huling Lyu1,3, Xianghong Luan1, Thomas G. H. Diekwisch1 1Center for Craniofacial Research and Diagnosis, Texas A&M College of Dentistry, 2Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, 3Key Laboratory of Oral Medicine, Guangzhou Insititute of Oral Disease, Stomatology Hospital of Guangzhou Medical University This protocol presents a method for the culture and 3D growth of ameloblast-like cells in microgravity to maintain their elongated and polarized shape as well as enamel-specific protein expression. Culture conditions for the culture of periodontal engineering constructs and lung organs in microgravity are also described.
