USDA-ARS Veterinary Pest Research Unit 1 article published in JoVE Biology Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles Brenda Leal-Galvan1, Cristina Harvey1, Donald Thomas2, Perot Saelao3, Adela S. Oliva Chavez1 1Department of Entomology, Texas A&M University, 2USDA-ARS Cattle Fever Tick Research Laboratory, 3USDA-ARS Veterinary Pest Research Unit The present protocol describes the isolation of microRNAs from tick salivary glands and purified extracellular vesicles. This is a universal procedure that combines commonly used reagents and supplies. The method also allows the use of a small number of ticks, resulting in quality microRNAs that can be readily sequenced.
