Virginia-Maryland College of Veterinary Medicine 2 articles published in JoVE Cancer Research Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model Margaret A. Nagai-Singer1, Alissa Hendricks-Wenger1,2,3, Rebecca M. Brock1,2, Holly A. Morrison1, Juselyn D. Tupik1, Sheryl Coutermarsh-Ott1, Irving C. Allen1,2,4 1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, 2Graduate Program in Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, 3Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, 4Department of Basic Science Education, Virginia Tech Carilion School of Medicine, Virginia Polytechnic Institute and State University We describe a more consistent and expeditious method to quantify lung metastasis in the 4T1 breast cancer model by using Fiji-ImageJ. Immunology and Infection The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids Daniel E. Rothschild1, Tara Srinivasan2, Linette A. Aponte-Santiago1, Xiling Shen2,3,4, Irving C. Allen1 1Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering, Cornell University, 3School of Electrical and Computer Engineering, Cornell University, 4Department of Biomedical Engineering, Duke University Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.
