Robert C. Byrd Biotechnology Science Center 1 article published in JoVE Immunology and Infection Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate Roy Al Ahmar*1, Brandon D. Kirby*2, Hongwei D. Yu1,2,3 1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.
