University of New England 4 articles published in JoVE Medicine Noninvasive Electrocardiography in the Perinatal Mouse Lindsey A. Fitzsimons1,2, Victoria L. Brewer3, John Forrester2, Adrian M. Moran4, Kerry L. Tucker1,2 1Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME, 2Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England. Biddeford, ME, 3College of Arts and Sciences, University of New England, Biddeford, ME, 4Department of Pediatrics, Division of Pediatric Cardiology, Maine Medical Center, Portland, ME Here, we present a noninvasive electrocardiography (ECG) protocol, optimized for early postnatal mice, that does not require the use of anesthetics. Chemistry Application of Voltage in Dynamic Light Scattering Particle Size Analysis Tianyu Ren*1, Emma J. Roberge*2, John R. Csoros1, W. Rudolph Seitz1, Eva Rose M. Balog3, Jeffrey Mark Halpern2 1Department of Chemistry, University of New Hampshire, 2Department of Chemical Engineering, University of New Hampshire, 3Department of Chemistry and Physics, University of New England Here, a protocol to apply voltage to solution during dynamic light scattering particle size measurements with the intent to explore the effect of voltage and temperature changes on polymer aggregation is presented. Neuroscience Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue Jennifer T. Malon1, Ling Cao1 1Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue. Biology Measurement of Larval Activity in the Drosophila Activity Monitor Aidan L. McParland1, Taylor L. Follansbee1, Geoffrey K. Ganter1 1Department of Biology, University of New England This report describes a method for measuring Drosophila larval activity using the TriKinetics Drosophila Activity Monitor. The device employs infrared beams to detect movements of up to 16 individual animals. Data can be analyzed to represent motion parameters including rates and the positions of the animals within the assay chambers.
