Royal Military College of Canada 1 article published in JoVE Environment Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites Mackenzie J. Denyes1, Michèle A. Parisien1, Allison Rutter2, Barbara A. Zeeb1 1Department of Chemistry and Chemical Engineering, Royal Military College of Canada, 2 Biochar is a carbon-rich material used as a soil amendment with the ability to sustainably sequester carbon, improve substrate quality and sorb contaminants. This protocol describes the 17 analytical methods used for the characterization of biochar, which is required prior to large scale implementation of these amendments in the environment.