Gift of Hope Organ and Tissue Donor Network 1 article published in JoVE Medicine A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft Michelle Mendiola Pla1, Amy Evans2, Franklin H. Lee3, Yuting Chiang1, Muath Bishawi1, Andrew Vekstein1, Lillian Kang1, Diego Zapata4, Ryan Gross3, Alexis Carnes2, Lynden E. Gault5, Julie A. Balko6, Desiree Bonadonna2, Sam Ho5, Paul Lezberg7, Benjamin S. Bryner1, Jacob N. Schroder1, Carmelo A. Milano1, Dawn E. Bowles3 1Division of Cardiothoracic Surgery, Department of Surgery, Duke University, 2Perfusion Services, Duke University, 3Division of Surgical Sciences, Department of Surgery, Duke University, 4Division of Laboratory Animal Resources, Duke University, 5Gift of Hope Organ and Tissue Donor Network, 6College of Veterinary Medicine, North Carolina State University, 7TransMedics, Inc We present a protocol for utilizing a normothermic ex vivo sanguinous perfusion system for the delivery of therapeutics to an entire cardiac allograft in a porcine heterotopic heart transplant model.
