Aptagen LLC 2 articles published in JoVE Bioengineering Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction Albert M. Liao1, Tran Thi Thanh Thoa1, G. Thomas Caltagirone1 1Aptagen LLC The present protocol demonstrates the use of a fast, 3-stage, aptamer-based exponential amplification assay to detect targets. Sample preparation, signal amplification, and color development are covered to implement this system to recognize the presence of theophylline over that of caffeine. Biochemistry Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry Tran Thi Thanh Thoa1, Albert M. Liao1, G. Thomas Caltagirone1 1Aptagen LLC The present protocol describes the use of Isothermal Titration Calorimetry (ITC) to analyze the association and dissociation kinetics of the binding between a DNA aptamer and tetracycline, including sample preparation, running standards and samples, and interpreting the resulting data.
